Hi, I am Xiaofei Zhang. Now I am doing post-doc at the Department of Agronomy and Plant Genetics in University of Minnesota.
I got my Ph.D. in the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences. During my PhD research, I focus on characterizing glutenin subunits, which are essential for determining the quality of wheat flour products, such as bread and noodles. Collaborating with the other two groups, I have identified and characterized low-molecular-weight glutenin subunits (LMW-GS) genes in an elite wheat variety (Xiaoyan 54) and the micro-core collections of Chinese wheat germplasm using proteomics and molecular genetics approaches.
1.Characterize LMW-GS in one variety
Investigate the components of LMW-GS in Xiaoyan54 (with elite bread-making quality) by using a proteomics-based approach, including 2D-PAGE, MALDI-TOF-MS, LC-MS/MS and database searching.
Ling-li Dong, Xiao-fei Zhang, Dong-cheng Liu, Hua-jie Fan, Jia-zhu Sun, Zhong-juan Zhang, Huan-ju Qin, Bin Li, Shan-ting Hao, Zhen-sheng Li, Dao-wen Wang, Ai-min Zhang, Hong-qing Ling (2010) New insights into the organization, recombination, expression and functional mechanism of low molecular weight glutenin subunit genes in bread wheat. PLoS ONE 5(10): e13548. doi:10.1371/journal.pone.0013548 (co-first author)
2. Separate LMW-GS genes in any variety
Establish a LMW-GS gene molecular marker system for separating LMW-GS genes (15-18) in individual wheat varieties.
Xiao-fei Zhang, Dong-cheng Liu, Wen-long Yang, Kun-fan Liu, Jia-zhu Sun, Xiao-li Guo, Yi-wen Li, Dao-wen Wang, Hong-qing Ling, Ai-min Zhang (2011) Development of a new marker system for dentifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.). Theoretical Applied Genetics 112：1503-1516
3. Identify full-lenth genes in any variety
Develop a PCR-based method for identifying full-length LMW-GS genes in wheat varieties.
Xiao-fei Zhang, Dong-cheng Liu, Wei Jiang, Xiao-li Guo, Wen-long Yang, Jia-zhu Sun, Hong-qing Ling, Ai-min Zhang. (2011) PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.). Theoretical Applied Genetics 123(8):1293-305
4. Dissecting the complex gene family in bread wheat
Investigate the micro-core collections of Chinese wheat germplasm by using the PCR-based method and the LMW-GS gene marker system.
Xiaofei Zhang, Dongcheng Liu, Jianghua Zhang, Wei Jiang, Guangbin Luo, Wenlong Yang, Jiazhu Sun, Yiping Tong, Dangqun Cui, Aimin Zhang. (2013) Novel insights into the composition, variation, organization and expression of the low-molecular-weight glutenin subunit gene family in common wheat. Journal of experimental Botany. (Accept)
5. Elucidating the function of low-molecular-weight glutenin alleles in bread wheat
Investigated 16 Aroona near-isogenic lines (NILs) to analyze the composition and function of LMW-GS alleles using SDS-PAGE, 2D-PAGE and the LMW-GS gene marker system.
Xiaofei Zhang, Hui Jin, Yan Zhang, Dongcheng Liu, Genying Li, Xianchun Xia, Zhonghu He, and Aimin Zhang. (2012) Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat. BMC Plant Biology, 12:243.
6. Denominating LMW-GS based on the nucleotide sequence polymorphism (in process)