Wheat Quality and Low-molecular-weight Glutenin Subunits (LMW-GS)

Hi, I am Xiaofei Zhang. Now I am doing post-doc at the Department of Agronomy and Plant Genetics in University of Minnesota.

I got my Ph.D. in the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences. During my PhD research, I focus on characterizing glutenin subunits, which are essential for determining the quality of wheat flour products, such as bread and noodles. Collaborating with the other two groups, I have identified and characterized low-molecular-weight glutenin subunits (LMW-GS) genes in an elite wheat variety (Xiaoyan 54) and the micro-core collections of Chinese wheat germplasm using proteomics and molecular genetics approaches.

1.Characterize LMW-GS in one variety 

Investigate the components of LMW-GS in Xiaoyan54 (with elite bread-making quality) by using a proteomics-based approach, including 2D-PAGE, MALDI-TOF-MS, LC-MS/MS and database searching.

Ling-li Dong, Xiao-fei Zhang, Dong-cheng Liu, Hua-jie Fan, Jia-zhu Sun, Zhong-juan Zhang, Huan-ju Qin, Bin Li, Shan-ting Hao, Zhen-sheng Li, Dao-wen Wang, Ai-min Zhang, Hong-qing Ling (2010) New insights into the organization, recombination, expression and functional mechanism of low molecular weight glutenin subunit genes in bread wheat. PLoS ONE 5(10): e13548. doi:10.1371/journal.pone.0013548 (co-first author)

2. Separate LMW-GS genes in any variety

Establish a LMW-GS gene molecular marker system for separating LMW-GS genes (15-18) in individual wheat varieties.

Xiao-fei Zhang, Dong-cheng Liu, Wen-long Yang, Kun-fan Liu, Jia-zhu Sun, Xiao-li Guo, Yi-wen Li, Dao-wen Wang, Hong-qing Ling, Ai-min Zhang (2011) Development of a new marker system for  dentifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.). Theoretical Applied Genetics 112:1503-1516

3. Identify full-lenth genes in any variety

Develop a PCR-based method for identifying full-length LMW-GS genes in wheat varieties.

Xiao-fei Zhang, Dong-cheng Liu, Wei Jiang, Xiao-li Guo, Wen-long Yang, Jia-zhu Sun, Hong-qing Ling, Ai-min Zhang. (2011) PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.). Theoretical Applied Genetics 123(8):1293-305

4. Dissecting the complex gene family in bread wheat

Investigate the micro-core collections of Chinese wheat germplasm by using the PCR-based method and the LMW-GS gene marker system.

Xiaofei Zhang, Dongcheng Liu, Jianghua Zhang, Wei Jiang, Guangbin Luo, Wenlong Yang, Jiazhu Sun, Yiping Tong, Dangqun Cui, Aimin Zhang. (2013) Novel insights into the composition, variation, organization and expression of the low-molecular-weight glutenin subunit gene family in common wheat. Journal of experimental Botany. (Accept)

5. Elucidating the function of low-molecular-weight glutenin alleles in bread wheat

 Investigated 16 Aroona near-isogenic lines (NILs) to analyze the composition and function of LMW-GS alleles using SDS-PAGE, 2D-PAGE and the LMW-GS gene marker system.

Xiaofei Zhang, Hui Jin, Yan Zhang, Dongcheng Liu, Genying Li, Xianchun Xia, Zhonghu He, and Aimin Zhang. (2012) Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat. BMC Plant Biology, 12:243.

6. Denominating LMW-GS based on the nucleotide sequence polymorphism (in process)

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4 Responses to “Wheat Quality and Low-molecular-weight Glutenin Subunits (LMW-GS)”

  1. Muhammad Sajjad March 7, 2013 at 1:41 am #

    Dear Dr.Zhand
    I am dr. Sajjad from university of Agriculture, Faisalabad. I am writing a research article on HMW, LMW and IB-1R translocation effect on bread making quality in wheat. Your PhD work is very impressive and therefore I am reading it thoroughly to get useful information for my write up. From your experience can you please comment on SDS-page validity to detect LMW-GS comared with 2D-PAGE, and other Proterozoic and molecular approaches? I shall try to stay in touch with you for useful communication.

    Profound regards
    Dr. sajjad

    • Xiaofei Zhang March 7, 2013 at 10:55 am #

      Dear Dr. Sajjad,

      Thank you for your comments. I’d like to share some information.

      SDS-PAGE is good for analyzing HMW-GS. But for LMW-GS, due to the large number of members and their similar molecular weight, it is a little difficult to identify different alleles if you do not have enough experience. 2-D gel works well for separate LMW-GS, but we need MS for identification followed by digested with Chymotrypsin.

      I prefer using the LMW-GS gene marker system (TAG, 2011) to identify LMW-GS genes in common wheat. It is useful to detect the gene members in individual varieties in details. The Glu-A3 and Glu-B3 markers developed by Wang (2010) might be helpful and easy to be use for allele identification. But I did not use them. I have no experience.

      Keep in touch.

      Thanks,
      Xiaofei

  2. Donald Kasarda July 12, 2013 at 8:49 pm #

    Can you tell me if the cultivar Xiaoyan 54 is a semi-dwarf or of normal height?

    • Xiaofei Zhang October 2, 2014 at 9:24 am #

      Xiaoyan54 is semi-dwarf. Thanks.

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